Isolation and Characterization of Dikaryotic Mutants from Pleurotus ostreatus by UV Irradiation

Protoplasts of the wild type strain of Pleurotus osteatus were mutagenized with UV light, and 3,000 colonies were examined for abnormal mycelial and fruiting phenotypes. Forty one strains displayed variant phenotypes in mycelia and fruiting processes. The variant phenotypes were classified into 6 groups: (1) auxotrophic strains, which are incapable of growing on minimal media and can only grow when provided with their specific requirements; (2) abnormal vegetative strains, which grow very slowly on minimal and complete media; (3) primordiumless strains, which fail to develop to the formation of primordia; (4) maturationless strains, which form primordia, but do not form mature fruiting bodies; (5) specifically colored strains, which have Specific bluish grey or bluish white pileus; (6) poorly spored strains, which fail to produce basidiospore or which produce few spores. These variant strains may be useful in genetic breeding programs and for the studies of fungal development and genetics.

Accuracy Tests of 3D Rapid Prototyping (RP) Medical Models: Its Potential and Clinical Applications

Presented in this paper are the experimental results that measure rapid prototyping (RP) errors in 3D medical models. We identified various factors that can cause dimensional errors when producing RP models, specifically in maxillofacial areas. For the experiment, we used a human dry skull. A number of linear measurements based on landmarks were first obtained on the skull. This was followed by CT scanning, 3D model reconstruction, and RP model fabrication. The landmarks were measured again on both the reconstructed models and the physical RP models, and these were compared with those on dry skull. We focused on major sources of errors, such as CT scanning, conversion from CT data to STL models, and RP model fabrication. The results show that the overall error from skull to RP is 0.64+/-0.36mm(0.71+/-0.66%) in absolute value. This indicates that the RP technology can be acceptable in the real clinical applications. A clinical case that has applied RP models successfully for treatment planning and surgical rehearsal is presented. Although the use of RP models is rare in the medical area yet, we believe RP is promising in that it has a great potential in developing new tools which can aid diagnosis, treatment planning, surgical rehearsal, education, and so on.

A Case of Vesical Endometriosis / 대한비뇨기과학회지

We report a case of vesical endometriosis in a 34 year old female patient and review literature. Managements include partial cystectomy, hormonal treatment and radiation. In this case, we select the hormonal treatrnent because the vesical endometriosis was in the fixed portion of the bladder.

Two Cases of Renal Vessel Malformation / 대한비뇨기과학회지

We have experienced two cases of renal vessel malformation caused by congenitally, one is extrarenal artery aneurysm with hypertension and the other is intrarenal arteriovenous fistula. We report two cases of renal vessel malformation and the literature has been reviewed.

A Clinical Observation on Urinary Tract Tuberculosis / 대한비뇨기과학회지

A clinical observation was made on 121 cases of urinary tract tuberculosis in the Department of Urology Hanyang University Hospital during the 8 year period from May, 1972 to April, 1980. The following results were obtained: 1. The incidence of urinary tract tuberculosis was 5.7% of total in-patients and the proportion of male to female was 1.7:1. 2. 34.4% were affected in the right kidney, 34.2% in the left and 31.4% in both. 3. Age and sex distribution showed that 33.9% were from 20 to 29 years old, 27.3% from 30 to 39 and 17.4% from 40 to 49. Regarding the sex, 76 cases (62.8%) were male and 45 cases (37.2%) were female. 4. Initial symptoms were mostly those of vesical irritable symptoms and hematuria (25.6%). 5. From past history 17.4% had pulmonary tuberculosis or pleurisy, 4.1% had tuberculous epididymitis, and 2.5% bone tuberculosis. 6. 25.6% of the cases visited the hospital within 1 month to 6 months after the onset of symptoms. 7. Other presenting active tuberculous lesions were pulmonary (14 cases), bony (3 cases) and lymphatic (1 case). 8. Ordinary urine cultures revealed mixed infection in 12 cases (15.2%), 4 cases of these 12 cases also had E. coli (5.1%). 9. A.F.B. was found in 67.5%. The total of all negative cases by the staining and culture method was 18.2%. 10. On cystoscopic findings 29 of 85 cases (34.1%) revealed a contracted bladder, but 5 cases (5.9%) showed normal finding. 11. Excretory urography showed calyceopelvic dilatation in 20.7% of cases, non-visualization in 19.3%, hydroureteronephrosis in 14.5%, contracted bladder in 12.4%, calcification in 7.6% and most cases had multiple changes in the kidneys. 12. Urographic findings based on the Lattimer classification showed far advanced tuberculous lesions in almost all cases. 13. Operative procedures were simple nephrectomy in 32 cases (69.6%), ureteroneocystostomy in 6.5% and ileocystoplasty with ureteroneocystostomy in 4.3% of 46 cases.